Alive healthy laboratory mouse obtained from animal laboratories that have not been inoculated with or exposed to any livestock or avian species is conducted or poultry disease agent’s exotic.
Species: Mouse
Strain: C57BL/6 or BALB/c
Sample Source: Bone Marrow
Selection Method: Negative, Untouched.
Application: Single-Cells Suspension Isolation.
Cell Type: Hematopoietic Stem and Progenitor Cells (HSPCs).
Markers: CD34low/-, SCA-1+, CD90/Thy1+/low, CD117+, c-Kit+, and Lin-
Characterization: Analysis of mouse hematopoietic stem cells by surface phenotype using Fluorescence-Activated Cell Sorting (FACS). Purity: >80%.

Fresh bone marrow is procured from the mouse femurs. The mononuclear fraction is then further processed by using lysis method to eliminate red blood cells and platelets. About 5-10 donor samples are pooled for filcolling. Ficoll-Paque PLUS method is used and washed the cells four times with RPMI prior to MNC collection.

Purity Assessment: Isolation of mouse hematopoietic stem and progenitor cells (HSPCs) from bone marrow consists of removing mature cells that express ‘lineage’ (Lin) antigens specific to terminally differentiated blood cells. Lineage antigens are absent or weakly expressed on HSPCs. HSPCs are positive for Sca1 (Ly-6A/E) and c-Kit (the receptor for SCF, also known as CD117) and referred to as LSK (Lin-Sca1+c-Kit+).1,2,3,4 LSK cells, which make up < 0.1% of nucleated BM cells, contain most repopulating stem cells but are depleted of more mature erythroid, myeloid, and megakaryoid cells including most colony-forming units (CFUs) which are Lin-Sca1-/lowc-Kit+.5 Mouse HSPCs are heterogeneous for other antigens such as CD34 and Thy1.

For purity assessment of these subsets of progenitor cells by flow cytometry use the following fluorochrome-conjugated antibody clones:
Anti-Mouse CD117 Antibody, Clone 2B8, and
Anti-Mouse Sca1 Antibody, Clone E13-161.7, and
Anti-mouse lineage-specific antibodies

For lineage-specific antigen labeling use the following fluorochrome-conjugated antibody clones:
Anti-Mouse CD3e Antibody, Clone 145-2C11, and
Anti-Mouse CD11b Antibody, Clone M1/70, and
Anti-Mouse CD19 Antibody, Clone 6D5, and
Anti-Mouse CD45R Antibody, Clone RA3-6B2, and
Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, and
Anti-Mouse TER119 Antibody, Clone TER-119

Mouse HSPCs can be used to study of Innate Immune Response, Stem Cell exhaustion, Infection, Inflammation, Pathogen, Innovation of Chronic diseases, Including Cancer, and May thus Offer Potential Treatment Options.

The cells are Non-Infectious, Non-Hazardous and for In vitro researches use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

FACS Data: