Alive healthy laboratory mouse obtained from animal laboratories that have not been inoculated with or exposed to any livestock or avian species is conducted or poultry disease agent’s exotic. The fresh spleen is harvested from alive mouse and dissociated from debris. Lysis method is used to eliminate red blood cells and platelets. About 10 donor samples are pooled for filcolling. Ficoll-Paque PLUS method is used and washed the cells four times with RPMI prior to performing cell isolation. CD11b+ microglia cells are isolated using specific immunomagnetic method with AutoMACS system following the protocol of the manufacturer. Purified of CD11b+ microglia cells are analyzed using a laser analytical flow cytometer, confirming the purity to be greater than 90%. FACScan will be provided.

Customize project with unit size, single or pooled donors and strains, C57BL/6, BALB/c, C3H, DBA/2, FVB, SJL, 129, DBA/1, ICR, KM, Swiss, and NIH are available upon request.

The cells are Non-Infectious, Non-Hazardous and for In vitro researches use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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