Alive healthy laboratory rat obtained from animal laboratories that have not been inoculated with or exposed to any livestock or avian species is conducted or poultry disease agent’s exotic. The fresh spleen is harvested from live rat and dissociated from debris. Lysis method is used to eliminate red blood cells and platelets to purify the splenocytes. About 5 donor samples are pooled for filcolling. Ficoll-Paque PLUS method is used and washed the cells four times with RPMI prior to collecting splenocytes. The splenocytes are enriched by density separation.

Rat Splenocytes can be used to study: Growth factor and cytokine release from lymphocytes, monocytes, and Kupffer cells, Function and mechanism of normal and diseased models in the reticuloendothelial system, Homing and processing of red blood cells, Antigen-recognition conditioning and proliferation of lymphocytes and monocytes, Reserve mechanisms for red blood cells and monocytes Purity.

The cells are Non-Infectious, Non-Hazardous and for In vitro researches use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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