NHP PB MNC are enriched by density separation. NHP BM MNC are isolated from fresh bone marrow by diluting the bone marrow with phosphate-buffered saline and using gradient separation techniques. After centrifugation, the MNC layer is collected. MNC can be processed to isolate subpopulations. NHP BM CD34+ cells are isolated from NHP bone marrow mononuclear cells using a direct immunomagnetic CD34+ MicroBead labeling system. Harvested purified cells are analyzed using a laser analytical flow cytometer confirming the purity is greater than 90%. FACScan will be provided. NHP-Plasma is processed through centrifugation to diminish cells and platelets.

NHP products can be used to study: Non-human primate lymphocyte and monocyte functions, Evolutionary conservation and homology between human and non-human primate lymphocytes and monocytes, The homology of BM-CD34+ cells between humans and non-human primates, especially M. fascicularis  (cynomolgus) monkeys, The hematopoietic stem cell potential of the non-human primate bone marrow CD34+ cells, Myeloid lineage differentiation and maturation, Neutrophil transplantation potential.

Cryopreservation and percentage used: 50% of Iscove’s DMEM with L-glutamine & 25 mM HEPES, 40% of Fetal Bovine Serum (FBS), 10% of Dimethyl sulfoxide (DMSO). 

Commonly used media and buffer in biological research, such like Staining Buffer (SB), Phosphate-Buffered Saline (PBS), Bovine Serum Albumin (BSA), RPMI (without L-glutamine) and Dulbecco’s Modified Eagle’s Medium (DMEM) might be used in the cells isolation processes. 

The NHP cells are Non-Infectious, Non-Hazardous and for researches use only. This product is for In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.